MOLECULAR SEXING AND ITS RELATIONSHIP WITH THE SEMI-CONCEALED YELLOW CORONAL PATCH OF THE WHITE-THROATED SPADEBILL PLATYRINCHUS MYSTACEUS CANCROMUS

Authors

DOI:

https://doi.org/10.58843/ornneo.v34i2.1237

Keywords:

Avian sexing, CHD gene, Passerine, Sex chromosomes, Sex-linked markers, Tyrannidae

Abstract

In sexually monomorphic species, sex identification by molecular techniques is an important alternative. In this study, we employed molecular sexing to determine the sex of seven adult females, two immature females, one female with missing age data, and 21 adult males of White-throated Spadebills Platyrinchus mystaceus cancromus. The sex and age of each individual were associated with the presence or absence of a semi-concealed yellow coronal patch, typical of the species. All males displayed the semi-concealed patch, and four out of seven adult females also exhibited it. One of the two juvenile females exhibited a patch. The female with missing age data also exhibited the patch. This finding disputes previous reports that juveniles of this species do not have coronal patches. We conclude that semi-concealed yellow coronal patches are not sexually dimorphic in White-throated Spadebills. Both sexes can have a patch, but adults who do not have them are females.

References

Alvares, CA, JL Stape, PC Sentelhas, JLM Gonçalves & G Sparovek (2013) Köppen’s climate classification map for Brazil. Meteorologische Zeitschrift 22: 711–728. https://doi.org/10.1127/0941-2948/2013/0507

An, J, MY Lee, M-S Min, M-H Lee & H Lee (2007) A molecular genetic approach for species identification of mammals and sex determination of birds in a forensic case of poaching from South Korea. Forensic Science International 167: 59–61. https://doi.org/10.1016/j.forsciint.2005.12.031

Cerit, H & K Avanus (2007) Sex identification in avian species using DNA typing methods. World's Poultry Science Journal 63: 91–100. https://doi.org/10.1017/S0043933907001316

del Hoyo, J, J Tello, GM Kirwan & N Collar (2020) White-throated Spadebill (Platyrinchus mystaceus), version 1.0. in Billerman, SM, BK Keeney, PG Rodewald & TS Schulenberg (eds). Birds of the World. Cornell Lab of Ornithology, Ithaca, NY, USA.

Donohue, KC & AM Dufty Jr (2006) Sex determination of Red-tailed Hawks (Buteo jamaicensis calurus) using DNA analysis and morphometrics. Journal of Field Ornithology 77: 74–79. https://doi.org/10.1111/j.1557-9263.2006.00003.x

Evans, DM & KR Day (2001) Migration patterns and sex ratios of diving ducks wintering in Northern Ireland with specific reference to Lough Neagh. Ringing & Migration 20: 358–363. https://doi.org/10.1080/03078698.2001.9674263

Gonzalez, SJ, JP Croxall & AG Wood (2000) Sexual dimorphism and sexual segregation in foraging strategies of northern Giant Petrels, Macronectes halli, during incubation. Oikos 90: 390–398. https://doi.org/10.1034/j.1600-0706.2000.900220.x

Griffiths, R, MC Double, K Orr & RJG Dawson (1998) A DNA test to sex most birds. Molecular Ecology 7: 1071–1075. https://doi.org/10.1046/j.1365-294x.1998.00389.x

Gwynne, JA, RS Ridgely, G Tudor & M Argel (2010) Aves do Brasil: Pantanal & Cerrado. Wildlife Conservation Society. Editora Horizonte, São Paulo, Brazil.

Kelly, JP & C Wood (1996) Diurnal, intraseasonal, and intersexual variation in foraging behavior of the Common Yellowthroat. The Condor 98: 491–500. https://doi.org/10.2307/1369563

Koch, HR, E Blohm‐Sievers & M Liedvogel (2019) Rapid sex determination of a wild passerine species using loop‐mediated isothermal amplification (LAMP). Ecology and Evolution 9: 5849–5858. https://doi.org/10.1002/ece3.5168

Macario, P, M Pichorim, PF Doherty Jr, GS Toledo-Lima, TM Oliveira-Júnior, TPF Câmara, SM Melo, JLS Silveira, JC Araújo & LF França (2017) Apparent survival and cost of reproduction for White-lined Tanager (Tachyphonus rufus, Thraupidae) in the northern Atlantic Rainforest, Brazil. PLoS ONE 12(10): e0185890. https://doi.org/10.1371/journal.pone.0185890

Noske, RA (2003) Sexual differences in the foraging behavior of Crested Shrike-Tits, Falcunculus frontatus, during winter. Emu 103: 271–277. https://doi.org/10.1071/MU03021

Ong, AH & S Vellayan (2008) An evaluation of CHD-specific primer sets for sex typing of birds from feathers. Zoo Biology: Published in affiliation with the American Zoo and Aquarium Association 27: 62–69. https://doi.org/10.1002/zoo.20163

Ridgely, RS, JA Gwynne, G Tudor & M Argel (2015) Aves do Brasil: Mata Atlântica do Sudeste. Wildlife Conservation Society. Editora Horizonte, São Paulo, Brazil.

Rodrigues, P, E Campos, J Micael & C Verdugo (2019) Sex determination of Neotropic cormorant (Phalacrocorax brasilianus) by molecular sexing. Avian Biology Research 12: 1–3. https://doi.org/10.1177/1758155919832130

Sambrook, J & DW Russell (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor, New York, USA.

Sanguinetti, CJ, EN Dias & AJ Simpson (1994) Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 17: 914–921.

Shibuya, FLS, FL Presti, SAC Lopes, PG Mota & JJ Roper (2018) Molecular Sex Determination in Neotropical Monochromatic Hummingbirds. Ornitología Neotropical 29: 225–228. https://doi.org/10.58843/ornneo.v29i1.344

Sick, H (1997) Ornitologia Brasileira. Nova Fronteira, Rio de Janeiro, Brazil.

Sigrist, T (2013) Avifauna brasileira: guia de campo Avis Brasilis. 3ª ed. Avis Brasilis Editora, Brazil.

Swengel, SR (1996) Special techniques, C: Sex determination. Pp. 223–231 in Ellis, DH, GF Gee & CM Mirande (eds). Cranes: Their Biology, Husbandry, and Conservation. National Biological Service/International Crane Foundation, USA.

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Published

21-11-2023

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Short Communications