The Effects of a Static Magnetic Field on the sensitivity of Escherichia coli to Ampicillin, Streptomycin and Nalidixic Acid



The goal of this study was to determine if we could increase the sensitivity of E. coli to antibiotics by exposing it to a static magnetic field (SMF). In this study, we exposed 12 mL aliquots of E. coil culture to a 19.5 mT SMF for 300-, 100-, 30-, or 10-minute intervals at 36 °C, then performed a disk diffusion assay using ampicillin, streptomycin and nalidixic acid. We looked for differences in the zones of inhibition (ZI) between SMF exposed and unexposed E. coli to quantify changes in antibiotic sensitivity. We found that exposure to SMF for 300 minutes results in a significantly larger ZI for ampicillin, and SMF exposure of 30-minutes reduced the ZI of streptomycin. Our results suggest that exposure to a 19.5 mT SMF can change E. coli’s susceptibility to ampicillin and streptomycin. This knowledge can be applied to developing an alternative treatment against infectious bacteria.


Hartwig V, Giovannetti G, Vanello N, Lombardi M, Landini L, Simi S. Biological Effects and Safety in Magnetic Resonance Imaging: A Review. Int J Environ Res Public Health 2009;6(6):2141-2144.

El May A, Snoussi S, Miloud N, Maatouk I, Abdelmelek H, Alssa R, et al., Effects of Static Magnetic field on Cell Growth, Viability, and Differential Gene Expression in Salmonella. Foodborne Pathogens and Disease 2009; 6(5):547-552.

Fojt, L., Strasak, L., Vetterl, V., & Smarda, J. (2004) Comparison of low frequency magnetic field effects on Escherichia coli, Lecericia adecarboxylata and Staphylococcus aureus. Biochemistry 63, 337-341.

Strasak L, Vetterl V, Smarda, J . Effects of low-frequency fields on bacteria Escherichia coli. Bioelectrochemistry 2002; 55(2):161-164.

Ji W, Huang H, Deng A, Pan C. Effects of low-frequency fields on bacteria Escherichia coli. Micron 2009; 40(8): 894-898.

Tagourti J, El May A, Aloui A, Chatti A, Aissa R, Landoulsi A . Static Magnetic field increases the sensitivity of Salmonella to gentamicin. Ann Mocrobiol 2010; 16(4):519-522.

Gaafar EA, Hanafy MS,Tohamy EY, Ibrahim MH. Stimulation and Control of E. coli by using an Extremely Low Frequency Magnetic Field. Romanian Journal of Biophysics 2006; 16(4):283-296.

Dickert H, Machka K, Braveny I. The uses and limitations of disc diffusion in the antibiotic sensitivity testing of bacteria. Infection. 1981;9(1):18-24.

Biswas D, Gorini L. The Attachment Site of Streptomycin to the 30S Ribosomal Subunit. Proceedings of the National Academy of Sciences. 1972;69(8):2141-2144.

Goss W, Dietz W, Cook T. Mechanism of Action of Nalidixic Acid on Escherichia coli II. Inhibition of Deoxyribonucleic Acid Synthesis. Journal of Bacteriology. 2016;89(4):1068-1074.

Dickert H, Machka K, Braveny I. The uses and limitations of disc diffusion in the antibiotic sensitivity testing of bacteria. Infection. 1981;9(1):18-24.

FilipiÄ J, Kraigher B, TepuÅ¡ B, Kokol V, Mandic-Mulec I. Effects of low-density static magnetic fields on the growth and activities of wastewater bacteria Escherichia coli and Pseudomonas putida. Bioresource Technology. 2012;120:225-232.

Kohno M, Yamazaki M, Kimura I, Wada M. Effect of static magnetic fields on bacteria: Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. Pathophysiology. 2000;7(2):143-148.

Luiciana D, Luigi A. Bioeffects of moderate-intensity static magnetic fields on cell cultures. Micron 2005; 36(3):195-217.

Binhi V, Alipov Y, Belyaev I. Effect of Static Magnetic Field on E. coli Cells and Individual Rotations of Ion Protein Complexes. Bioelectromagnetics 2002; 22(2):79-86.