BIOACTIVITY-GUIDED ISOLATION OF ANTICANCER AGENTS FROM BAUHINIA KOCKIANA KORTH.

Authors

  • Yik Ling Chew Faculty of Pharmaceutical Sciences, UCSI University
  • Yau Yan Lim School of Science, Monash University Sunway Campus
  • Johnson Stanlas Pharmacotherapeutics Unit, Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia
  • Gwendoline Cheng Lian Ee Department of Chemistry, Faculty of Science, Universiti Putra Malaysia
  • Joo Kheng Goh School of Science, Monash University Sunway Campus

Keywords:

Alternative Medicines, Traditional Medicines, Anticancer

Abstract

Background: Flowers of Bauhinia kockiana were investigated for their anticancer properties. Methods: Gallic acid (1), and methyl gallate (2), were isolated via bioassay-directed isolation, and they exhibited anticancer properties towards several cancer cell lines, examined using MTT cell viability assay. Pyrogallol (3) was examined against the same cancer cell lines to deduce the bioactive functional group of the phenolic compounds. Results: The results showed that the phenolic compounds could exhibit moderate to weak cytotoxicity towards certain cell lines (GI50 30 - 86 µM), but were inactive towards DU145 prostate cancer cell (GI50 > 100 µM). Conclusion: It was observed that pyrogallol moiety was one of the essential functional structures of the phenolic compounds in exhibiting anticancer activity. Also, the carboxyl group of compound 1 was also important in anticancer activity. Examination of the PC-3 cells treated with compound 1 using fluorescence microscopy showed that PC-3 cells were killed by apoptosis.

Author Biographies

Yik Ling Chew, Faculty of Pharmaceutical Sciences, UCSI University

Lecturer at Faculty of Pharmaceutical Sciences

Yau Yan Lim, School of Science, Monash University Sunway Campus

Associate Professor at School of Science, Monash University Sunway Campus

Johnson Stanlas, Pharmacotherapeutics Unit, Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia

Professor at the Department of Medicine, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia

Gwendoline Cheng Lian Ee, Department of Chemistry, Faculty of Science, Universiti Putra Malaysia

Professor at Department of Chemistry, Faculty of Science, Universiti Putra Malaysia

Joo Kheng Goh, School of Science, Monash University Sunway Campus

Senior Lecturer at School of Science, Monash University Sunway Campus

Downloads

Published

2014-03-29

How to Cite

Chew, Y. L., Lim, Y. Y., Stanlas, J., Ee, G. C. L., & Goh, J. K. (2014). BIOACTIVITY-GUIDED ISOLATION OF ANTICANCER AGENTS FROM BAUHINIA KOCKIANA KORTH. African Journal of Traditional, Complementary and Alternative Medicines, 11(3), 291–299. Retrieved from https://journals.athmsi.org/index.php/ajtcam/article/view/2007

Issue

Vol. 11 No. 3 (2014)

Section

Research Papers

License

Copyright: Creative Commons Attribution CC.

This license lets others distribute, remix, tweak, and build upon your work, even commercially, as long as they credit you for the original creation. This is the most accommodating of licenses offered. Recommended for maximum dissemination and use of licensed materials. View License Deed | View Legal Code Authors can also self-archive their manuscripts immediately and enable public access from their institution's repository. This is the version that has been accepted for publication and which typically includes author-incorporated changes suggested during submission, peer review and in editor-author communications.